Robert C Shields

Robert C Shields,

CO RES AST PROF

Department: DN-ORAL BIOLOGY
Business Phone: (352) 273-8850
Business Email: rshields@dental.ufl.edu

About Robert C Shields

I am currently a Research Assistant Professor in the Department of Oral Biology at the University of Florida. Prior to this, I was a postdoc also at UF, in Robert Burne’s lab working on cell-cell communication and regulation of gene expression in Streptococcus mutans. I received my PhD in Microbiology at Newcastle University (United Kingdom) in Nick Jakubovics lab, where I worked on extracellular DNA in microbial biofilms, and potential control of biofilms with eDNA-degrading enzymes.

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0002-8823-9504

Publications

2020
Peptides encoded in the Streptococcus mutans RcrRPQ operon are essential for thermotolerance
Microbiology. 166(3):306-317 [DOI] 10.1099/mic.0.000887. [PMID] 31935187.
2020
Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function
PLOS Pathogens. 16(3) [DOI] 10.1371/journal.ppat.1008344. [PMID] 32150575.
2019
Carbohydrate and PepO control bimodality in competence development by Streptococcus mutans.
Molecular microbiology. 112(5):1388-1402 [DOI] 10.1111/mmi.14367. [PMID] 31403729.
2019
Erratum for Underhill et al., “Intracellular Signaling by the comRS System in Streptococcus mutans Genetic Competence”
mSphere. 4(1) [DOI] 10.1128/msphere.00042-19.
2019
Fluorescence Tools Adapted for Real-Time Monitoring of the Behaviors of Streptococcus Species
Applied and Environmental Microbiology. 85(15) [DOI] 10.1128/aem.00620-19.
2019
Fluorescence Tools Adapted for Real-Time Monitoring of the Behaviors ofStreptococcusSpecies
Applied and Environmental Microbiology. 85(15) [DOI] 10.1128/aem.00620-19.
2019
Long-term fish assemblages of the Ohio River: Altered trophic and life history strategies with hydrologic alterations and land use modifications.
PloS one. 14(4) [DOI] 10.1371/journal.pone.0211848. [PMID] 31017895.
2019
The bare necessities: Uncovering essential and condition‐critical genes with transposon sequencing
Molecular Oral Microbiology. 34(2):39-50 [DOI] 10.1111/omi.12256.
2018
Competence inhibition by the XrpA peptide encoded within thecomXgene ofStreptococcus mutans
Molecular Microbiology. 109(3):345-364 [DOI] 10.1111/mmi.13989. [PMID] 29802741.
2018
Genome-Wide Screens Reveal New Gene Products That Influence Genetic Competence in Streptococcus mutans
Journal of Bacteriology. 200(2) [DOI] 10.1128/jb.00508-17.
2018
Genomewide Identification of Essential Genes and Fitness Determinants of Streptococcus mutans UA159
mSphere. 3(1) [DOI] 10.1128/msphere.00031-18.
2018
Genomewide Identification of Essential Genes and Fitness Determinants of Streptococcus mutans UA159
mSphere. 3(1) [DOI] 10.1128/msphere.00031-18.
2018
Intracellular Signaling by the comRS System in Streptococcus mutans Genetic Competence
mSphere. 3(5) [DOI] 10.1128/msphere.00444-18.
2018
Intracellular Signaling by thecomRSSystem inStreptococcus mutansGenetic Competence
mSphere. 3(5) [DOI] 10.1128/msphere.00444-18.
2017
A new arginine-based dental adhesive system: formulation, mechanical and anti-caries properties.
Journal of dentistry. 63:72-80 [DOI] 10.1016/j.jdent.2017.05.024. [PMID] 28587978.
2017
Genome-Wide Screens Reveal New Gene Products That Influence Genetic Competence in Streptococcus mutans
Journal of Bacteriology. 200(2) [DOI] 10.1128/jb.00508-17.
2017
Intercellular Communication via the comX-Inducing Peptide (XIP) of Streptococcus mutans.
Journal of bacteriology. 199(21) [DOI] 10.1128/JB.00404-17. [PMID] 28808131.
2016
Growth of Streptococcus mutans in Biofilms Alters Peptide Signaling at the Sub-population Level.
Frontiers in microbiology. 7 [DOI] 10.3389/fmicb.2016.01075. [PMID] 27471495.
2015
Bidirectional signaling in the competence regulatory pathway of Streptococcus mutans.
FEMS microbiology letters. 362(19) [DOI] 10.1093/femsle/fnv159. [PMID] 26363019.
2015
Conserved and divergent functions of RcrRPQ in Streptococcus gordonii and S. mutans.
FEMS microbiology letters. 362(16) [DOI] 10.1093/femsle/fnv119. [PMID] 26229070.
Carbohydrate and PepO control bimodality in competence development byStreptococcus mutans
. [DOI] 10.1101/670752.
Repurposing theStreptococcus mutansCRISPR-Cas9 System to Understand Essential Gene Function
. [DOI] 10.1101/791483.

Grants

Jul 2021 ACTIVE
Exploring Integrative Conjugative Elements in the Biology and Ecology of Oral Streptococci
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Mar 2021 ACTIVE
Assessing the impact of Agr quorum sensing on Staphylococcus aureus physiology in the space flight environment
Role: Co-Investigator
Funding: NASA KENNEDY SPACE FLIGHT CTR
Jul 2020 – Aug 2021
Development of new anti-caries therapeutics using a CRISPR interference platform
Role: Principal Investigator
Funding: COLGATE-PALMOLIVE CO
Aug 2018 ACTIVE
Probing the effect of simulated microgravity on the pathogenic potential of cariogenic Streptococcus mutans
Role: Co-Investigator
Funding: NASA STENNIS SPACE FLIGHT CTR

Education

PhD (Microbiology)
2010-2014 · Newcastle University (United Kingdom)
BSc (Marine Biology)
2006-2009 · Newcastle University (United Kingdom)

Contact Details

Phones:
Business:
(352) 273-8850
Emails: