L J Brady

L J Brady,

Professor & Director Of Graduate And Post-Doctoral Research Training And Career Development

Department: DN-ORAL BIOLOGY
Business Phone: (352) 273-8839
Business Email: jbrady@dental.ufl.edu

Teaching Profile

Courses Taught
2006,2010-2012,2012-2018,2016-2021,2018-2021,2020-2021
GMS6040 Host-Pathogen Interactions
2011-2012,2014-2021,2020-2022
DEN6128 Host Defense
2011-2022
DEN5127 Infectious Diseases
2010,2013-2016,2016-2019,2018-2019
DEN8290 Special Topics
2018
GMS7980 Research for Doctoral Dissertation
2007,2009-2016
GMS6193 Research Conference in Oral Biology
2014
GMS6121 Infectious Diseases
2021
GMS6162 Oral Microbiology and Immunology

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0001-8952-086X

Publications

2021
Amyloid Aggregation of Streptococcus mutans Cnm Influences Its Collagen-Binding Activity
Applied and Environmental Microbiology. [DOI] 10.1128/aem.01149-21.
2021
Multiple factors are involved in regulation of extracellular membrane vesicle biogenesis in Streptococcus mutans
Molecular Oral Microbiology. [DOI] 10.1111/omi.12318. [PMID] 33040492.
2021
Protein Interactomes of Streptococcus mutans YidC1 and YidC2 Membrane Protein Insertases Suggest SRP Pathway-Independent- and -Dependent Functions, Respectively
mSphere. [DOI] 10.1128/msphere.01308-20.
2021
The Cytoplasmic Domains of Streptococcus mutans Membrane Protein Insertases YidC1 and YidC2 Confer Unique Structural and Functional Attributes to Each Paralog.
Frontiers in microbiology. [DOI] 10.3389/fmicb.2021.760873. [PMID] 34795653.
2020
Characterization of an intermolecular quaternary interaction between discrete segments of the Streptococcus mutans adhesin P1 by NMR spectroscopy
The FEBS Journal. [DOI] 10.1111/febs.15158. [PMID] 31782893.
2020
Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids.
Scientific reports. [DOI] 10.1038/s41598-020-62115-7. [PMID] 32198417.
2020
The Impacts of Sortase A and the 4′-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis.
Frontiers in microbiology. [DOI] 10.3389/fmicb.2020.570219. [PMID] 33193163.
2019
Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties
Journal of Bacteriology. [DOI] 10.1128/jb.00396-18.
2019
Membrane proteomic analysis reveals overlapping and independent functions of Streptococcus mutans Ffh, YidC1, and YidC2.
Molecular oral microbiology. [DOI] 10.1111/omi.12261. [PMID] 31034136.
2019
The Biology of Streptococcus mutans.
Microbiology spectrum. [DOI] 10.1128/microbiolspec.GPP3-0051-2018. [PMID] 30657107.
2018
Biology of Oral Streptococci.
Microbiology spectrum. [DOI] 10.1128/microbiolspec.GPP3-0042-2018. [PMID] 30338752.
2018
Characterization of the pgf operon involved in the posttranslational modification of Streptococcus mutans surface proteins.
Scientific reports. [DOI] 10.1038/s41598-018-23170-3. [PMID] 29549320.
2017
Functional amyloids in Streptococcus mutans, their use as targets of biofilm inhibition and initial characterization of SMU_63c.
Microbiology (Reading, England). [DOI] 10.1099/mic.0.000443. [PMID] 28141493.
2017
K+ Modulates Genetic Competence and the Stress Regulon of Streptococcus Mutans
Microbiology. [DOI] 10.1099/mic.0.000458. [PMID] 28530170.
2017
LT adjuvant modulates epitope specificity and improves the efficacy of murine antibodies elicited by sublingual vaccination with the N-terminal domain of Streptococcus mutans P1.
Vaccine. [DOI] 10.1016/j.vaccine.2017.11.007. [PMID] 29146379.
2016
Evaluation of the effects of Streptococcus mutans chaperones and protein secretion machinery components on cell surface protein biogenesis, competence, and mutacin production.
Molecular oral microbiology. [DOI] 10.1111/omi.12130. [PMID] 26386361.
2016
Specific binding of a naturally occurring amyloidogenic fragment of Streptococcus mutans adhesin P1 to intact P1 on the cell surface characterized by solid state NMR spectroscopy.
Journal of biomolecular NMR. [DOI] 10.1007/s10858-016-0017-1. [PMID] 26837620.
2016
Trk2 Potassium Transport System in Streptococcus mutans and Its Role in Potassium Homeostasis, Biofilm Formation, and Stress Tolerance.
Journal of bacteriology. [DOI] 10.1128/JB.00813-15. [PMID] 26811321.
2015
Binding forces of Streptococcus mutans P1 adhesin.
ACS nano. [DOI] 10.1021/nn5058886. [PMID] 25671413.
2015
Breaking the bacterial protein targeting and translocation model: oral organisms as a case in point.
Molecular oral microbiology. [DOI] 10.1111/omi.12088. [PMID] 25400073.
2015
Identification of a supramolecular functional architecture of Streptococcus mutans adhesin P1 on the bacterial cell surface.
The Journal of biological chemistry. [DOI] 10.1074/jbc.M114.626663. [PMID] 25666624.
2014
Alterations in immunodominance of Streptococcus mutans AgI/II: lessons learned from immunomodulatory antibodies.
Vaccine. [DOI] 10.1016/j.vaccine.2013.11.023. [PMID] 24252705.
2014
An intramolecular lock facilitates folding and stabilizes the tertiary structure of Streptococcus mutans adhesin P1.
Proceedings of the National Academy of Sciences of the United States of America. [DOI] 10.1073/pnas.1413018111. [PMID] 25331888.
2014
Immunogenicity and in vitro and in vivo protective effects of antibodies targeting a recombinant form of the Streptococcus mutans P1 surface protein.
Infection and immunity. [DOI] 10.1128/IAI.02074-14. [PMID] 25225243.
2014
Streptococcus mutans extracellular DNA is upregulated during growth in biofilms, actively released via membrane vesicles, and influenced by components of the protein secretion machinery.
Journal of bacteriology. [DOI] 10.1128/JB.01493-14. [PMID] 24748612.
2014
YlxM is a newly identified accessory protein that influences the function of signal recognition particle pathway components in Streptococcus mutans.
Journal of bacteriology. [DOI] 10.1128/JB.01465-13. [PMID] 24659773.
2013
An intramolecular interaction involving the N terminus of a streptococcal adhesin affects its conformation and adhesive function.
The Journal of biological chemistry. [DOI] 10.1074/jbc.M113.459974. [PMID] 23539625.
2012
Brpa Is Involved in Regulation of Cell Envelope Stress Responses in Streptococcus Mutans
Applied and Environmental Microbiology. [DOI] 10.1128/AEM.07823-11. [PMID] 22327589.
2012
Functional amyloid formation by Streptococcus mutans.
Microbiology (Reading, England). [DOI] 10.1099/mic.0.060855-0. [PMID] 23082034.
2012
YidC1 and YidC2 are functionally distinct proteins involved in protein secretion, biofilm formation and cariogenicity of Streptococcus mutans.
Microbiology (Reading, England). [DOI] 10.1099/mic.0.059139-0. [PMID] 22504439.
2011
A therapeutic anti-Streptococcus mutans monoclonal antibody used in human passive protection trials influences the adaptive immune response.
Vaccine. [DOI] 10.1016/j.vaccine.2011.06.027. [PMID] 21704107.
2011
Crystal structure of the C-terminal region of Streptococcus mutans antigen I/II and characterization of salivary agglutinin adherence domains.
The Journal of biological chemistry. [DOI] 10.1074/jbc.M111.231100. [PMID] 21505225.
2010
Elongated fibrillar structure of a streptococcal adhesin assembled by the high-affinity association of alpha- and PPII-helices.
Proceedings of the National Academy of Sciences of the United States of America. [DOI] 10.1073/pnas.0912293107. [PMID] 20231452.
2010
Induction of neutralizing antibodies in mice immunized with an amino-terminal polypeptide of Streptococcus mutans P1 protein produced by a recombinant Bacillus subtilis strain.
FEMS immunology and medical microbiology. [DOI] 10.1111/j.1574-695X.2010.00669.x. [PMID] 20402772.
2010
The changing faces of Streptococcus antigen I/II polypeptide family adhesins.
Molecular microbiology. [DOI] 10.1111/j.1365-2958.2010.07212.x. [PMID] 20497507.
2009
Beneficial immunomodulation by Streptococcus mutans anti-P1 monoclonal antibodies is Fc independent and correlates with increased exposure of a relevant target epitope.
Journal of immunology (Baltimore, Md. : 1950). [DOI] 10.4049/jimmunol.0803300. [PMID] 19752237.
2009
Independent gene duplications of the YidC/Oxa/Alb3 family enabled a specialized cotranslational function.
Proceedings of the National Academy of Sciences of the United States of America. [DOI] 10.1073/pnas.0809951106. [PMID] 19366667.
2008
Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva.
Infection and immunity. [DOI] 10.1128/IAI.00422-08. [PMID] 18625741.
2008
Functional overlap but lack of complete cross-complementation of Streptococcus mutans and Escherichia coli YidC orthologs.
Journal of bacteriology. [DOI] 10.1128/JB.01366-07. [PMID] 18178746.
2008
Isolation and solubilization of cellular membrane proteins from bacteria.
Methods in molecular biology (Clifton, N.J.). [DOI] 10.1007/978-1-60327-210-0_23. [PMID] 18369904.
2008
Requirements for surface expression and function of adhesin P1 from Streptococcus mutans.
Infection and immunity. [DOI] 10.1128/IAI.01315-07. [PMID] 18362133.
2007
Basis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1.
FEMS immunology and medical microbiology. [PMID] 17614961.
2007
Characterization of epitopes recognized by anti-Streptococcus mutans P1 monoclonal antibodies.
FEMS immunology and medical microbiology. [PMID] 17535300.
2007
Consequences of a sortase A mutation in Streptococcus gordonii.
Microbiology (Reading, England). [DOI] 10.1099/mic.0.2007/007252-0. [PMID] 18048922.
2007
Membrane composition changes and physiological adaptation by Streptococcus mutans signal recognition particle pathway mutants.
Journal of bacteriology. [PMID] 17085548.
2006
A unique serine-rich repeat protein (Srr-2) and novel surface antigen (epsilon) associated with a virulent lineage of serotype III Streptococcus agalactiae.
Microbiology (Reading, England). [DOI] 10.1099/mic.0.28516-0. [PMID] 16549667.
2006
A whole cell BIAcore assay to evaluate P1-mediated adherence of Streptococcus mutans to human salivary agglutinin and inhibition by specific antibodies.
Journal of microbiological methods. [PMID] 16239043.
2005
Antibody-mediated immunomodulation: a strategy to improve host responses against microbial antigens.
Infection and immunity. [PMID] 15664904.
2005
Solubilization of cellular membrane proteins from Streptococcus mutans for two-dimensional gel electrophoresis.
Electrophoresis. [PMID] 15706571.
2005
Streptococcal viability and diminished stress tolerance in mutants lacking the signal recognition particle pathway or YidC2.
Proceedings of the National Academy of Sciences of the United States of America. [PMID] 16293689.
2004
An ffh mutant of Streptococcus mutans is viable and able to physiologically adapt to low pH in continuous culture.
FEMS microbiology letters. [PMID] 15135539.
2004
Characterization of the Streptococcus mutans P1 epitope recognized by immunomodulatory monoclonal antibody 6-11A.
Infection and immunity. [PMID] 15271929.
2004
Contribution of the alanine-rich region of Streptococcus mutans P1 to antigenicity, surface expression, and interaction with the proline-rich repeat domain.
Infection and immunity. [PMID] 15271931.
2004
Further characterization of immunomodulation by a monoclonal antibody against Streptococcus mutans antigen P1.
Infection and immunity. [PMID] 14688075.
2004
Redirecting the humoral immune response against Streptococcus mutans antigen P1 with monoclonal antibodies.
Infection and immunity. [PMID] 15557617.
2003
Characterization of group B streptococcal glyceraldehyde-3-phosphate dehydrogenase: surface localization, enzymatic activity, and protein-protein interactions.
Canadian journal of microbiology. [PMID] 12897829.
2001
Characterization of the sat operon in Streptococcus mutans: evidence for a role of Ffh in acid tolerance.
Journal of bacteriology. [PMID] 11274114.
2001
The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis.
Oral microbiology and immunology. [PMID] 11358537.
2000
IVIAT: a novel method to identify microbial genes expressed specifically during human infections.
Trends in microbiology. [PMID] 10878769.
2000
Monoclonal antibody-mediated modulation of the humoral immune response against mucosally applied Streptococcus mutans.
Infection and immunity. [PMID] 10722566.
1999
Streptococcus mutans ffh, a gene encoding a homologue of the 54 kDa subunit of the signal recognition particle, is involved in resistance to acid stress.
Microbiology (Reading, England). [DOI] 10.1099/13500872-145-2-357. [PMID] 10075418.
1999
Virulence of a spaP mutant of Streptococcus mutans in a gnotobiotic rat model.
Infection and immunity. [PMID] 10024561.
1998
Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule.
Infection and immunity. [PMID] 9712778.
1996
Oral diseases, mycology and periodontal microbiology of HIV-1-infected women.
Oral microbiology and immunology. [PMID] 9467369.
1993
Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans.
Infection and immunity. [PMID] 8454362.
1992
Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1.
Infection and immunity. [PMID] 1541515.
1992
Molecular, immunological and functional characterization of the major surface adhesin of Streptococcus mutans.
Advances in experimental medicine and biology. [PMID] 1295342.
1991
Group B streptococcal C protein-associated antigens: association with neonatal sepsis.
The Journal of infectious diseases. [PMID] 2010631.
1991
Identification of monoclonal antibody-binding domains within antigen P1 of Streptococcus mutans and cross-reactivity with related surface antigens of oral streptococci.
Infection and immunity. [PMID] 1937801.
1991
Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates.
Infection and immunity. [PMID] 1673448.
1990
Cloning and inactivation of the gene responsible for a major surface antigen on Streptococcus mutans.
Archives of oral biology. [PMID] 2088219.
1990
Colloidal gold immunolabeling of immunoglobulin-binding sites and beta antigen in group B streptococci.
Infection and immunity. [PMID] 2404866.
1989
Identification of non-immunoglobulin A-Fc-binding forms and low-molecular-weight secreted forms of the group B streptococcal beta antigen.
Infection and immunity. [PMID] 2651313.
1988
Two novel antigens associated with group B streptococci identified by a rapid two-stage radioimmunoassay.
The Journal of infectious diseases. [PMID] 3053930.
Amyloid aggregation of Streptococcus mutans Cnm influences its collagen-binding activity
. [DOI] 10.1101/2021.06.09.447825.
Protein Interactomes Identify Distinct Pathways forStreptococcus mutansYidC1 and YidC2 Membrane Protein Insertases
. [DOI] 10.1101/2020.04.07.031013.

Grants

Jul 2022 ACTIVE
MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jan 2022 ACTIVE
Characterization and membrane-biogenesis of Streptococcus mutans magnesium transporters
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDCR
Jun 2020 – Jun 2022
NMR console upgrade for structural biology and metabolomics
Role: Faculty
Funding: NATL INST OF HLTH OD
Jul 2019 ACTIVE
Intracellular Invasion by Streptococcus mutans: Significance in Disease
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDCR
Mar 2017 ACTIVE
Functional amyloid formation in streptococcus mutans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jul 2016 – Jun 2021
Comprehensive Training Program in Oral Biology
Role: Other
Funding: NATL INST OF HLTH NIDCR
Jul 2016 – Jun 2021
Comprehensive Training Program in Oral Biology
Role: Other
Funding: NATL INST OF HLTH NIDCR
Apr 2016 – Mar 2019
eDNA secretion and eDMA-cell interactions in biofilm formation
Role: Principal Investigator
Funding: LOUISIANA STATE UNIV via NATL INST OF HLTH
Dec 2015 – Jun 2022
MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Mar 2012 – Feb 2017
Functional Amyloid Formation in Streptococcus mutans
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR
Jul 2009 – Nov 2015
MEMBRANES OF THE DENTAL PATHOGEN STREPTOCOCCUS MUTANS
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDCR

Contact Details

Phones:
Business:
(352) 273-8839
Emails:
Addresses:
Business Mailing:
1395 CENTER DR
GAINESVILLE FL 32610
Business Street:
D4-22C
GAINESVILLE FL 32610