Links
- Electronic Research Administration
- Human Oral Microbiome Database
- National Institute of Dental and Craniofacial Research
- Oral Pathogen Sequence Databases
- PubMed
- University of Florida Emerging Pathogens Institute
Oral Biology Department Resources
Overall Services
The Oral Biology Shared Resources Facility provides access to technologies, products, services, and expertise that facilitate interaction between researchers and enhance scientific productivity.
More importantly, these shared resources help avoid wasteful duplication of efforts; provide economies of scale that result in cost savings; facilitate access to expensive equipment and highly skilled technical services; maintain quality control and consistency of service; and promote multidisciplinary interactions and collaborations among the research laboratory staff.
In addition to providing members access to state-of-the-art instrumentation and methodology, the shared resources facilitate education and technology transfer by holding equipment consultations, workshops, instrument demos, and offers scientific vendor discounts for our members.
In support of our endless strive for laboratory research excellence, we have incorporated the equipment scheduling software known as BookitLab to manage all shared instrument usage. As advocates of progress and creativity, our department understands the importance of visual representation in scientific endeavors.
To support the academic community, the Oral Biology Department’s Shared Resources Facility is thrilled to offer BioRender, a cutting-edge scientific graphic software, designed to revolutionize the way the faculty and students communicate their research findings.
For any questions, don’t hesitate to get in touch with our shared resources laboratory manager: Alexander Linares
Attune NxT – Flow Cytometer
Almost any standard flow cytometry application can benefit from the high sensitivity and throughput of the Attune NxT Flow Cytometer. See our Sample Data page for examples spanning infectious disease research, fluorescent proteins, CRISPR gene editing, immuno-oncology research, microbiology, plant ploidy, platelets, stem cells, and T cells.
One classic flow cytometry application is immunophenotyping. Flow cytometry is the method of choice for identifying cells within complex heterogeneous populations, as it allows for multiparameter analysis of thousands to millions of cells in a short time. Strong signal separation in the Attune NxT Flow Cytometer shows excellent separation of cell populations into subsets for immunophenotyping. A wide range of reagent choices—as well as the system’s automated compensation module, four spatially separated lasers, and 14 color choices—help to simplify multicolor panel design.
Bioscreen C MBR
Bioscreen C is a system designed for automating routine microbiology work. It is a computer-controlled incubator/ reader/ shaker equipped with 8 filters from 405nm to 600nm including a wide band filter. It has a sensitive reader measuring turbidity, a technique more sensitive than a spectrophotometer. Its unique patented incubator system can maintain temperature with a 0,1 degree centigrade accuracy. With the help of newly released software OD values can easily be modified into virtually any data or graph format without having to export raw data to third-party software solutions.
CFX96 Touch Real-Time PCR System S1000 Touch Thermal Cycler
The Bio-Rad CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions.
Quickly set up runs and monitor amplification traces in real-time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR.
Epoch 2 Microplate Spectrophotometer
The Agilent BioTek Epoch 2 microplate spectrophotometer delivers excellent performance for UV-Vis absorbance measurements. Measurements can be performed in 6- to 384-well microplates, cuvettes, and microvolume samples with the available Take3 microvolume plate. The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays with higher wavelengths.
Epoch 2 is easy to use and the optional touchscreen interface makes it simple to choose predefined protocols or to define custom programs. Endpoint, kinetic, spectral scanning, and well area scanning modes, plus incubation and shaking enable wide-ranging applications.
EVOS™ M7000 Imaging System with onstage incubator
This system has been designed with advanced capabilities to simplify demanding cell-based imaging applications such as live-cell analysis, image tiling, and Z-stacking, so you can focus on acquiring images and data rather than instrument operation.
When combined with the onstage incubation system, the EVOS M7000 Imaging System is ideal for long-term monitoring of cell cultures and time-lapse imaging at high resolution. The Invitrogen™ EVOS™ Onstage Incubator is an environmental chamber that allows for precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and nonphysiological conditions, making the system ideal for demanding hypoxia experiments. The EVOS Onstage Incubator allows you to:
• Intuitively set environmental and image-acquisition parameters
• Easily maintain physiological or nonphysiological conditions with precise control
• Adjust environmental parameters while the experiment is running
• Helps save lab space with a small footprint and sleek design
EVOS™ XL Core
Imaging System for Transmitted Light Applications The EVOS™ XL Core Imaging System has two types of controls: manual and onscreen. Manual controls include the illumination wheel, focus knobs, Freeze and Save buttons, objective turret, and the optional mechanical stage X-Y axis knobs. Onscreen controls appear at the bottom of the screen when the mouse rolls over the controls area. Using a mouse is optional; you can operate the EVOS™ XL Core Imaging System using only manual controls.
GatorPrime
Gator® Prime is our entry-level biolayer interferometry (BLI) instrument for label-free analysis.
With 8 independent spectrometers capable of running 8 samples at once, this instrument allows for assays sizes from 1 sample to 168 samples in one run. Flexible to accommodate various applications including quantitation, kinetics, and epitope binning. Easy to use and operate, this is an instrument that everyone in the laboratory can know how to run. This is truly a plug-and-play instrument, taking less than 30 minutes to set up and install.
Highlights
1. From 1-168 samples/ run
2. 10 x 10 epitope binning
3. Temperature controlled experiments
4. Quantitation and kinetics in one run
Luminex Magpix
The Magpix is a versatile multiplexing platform capable of performing qualitative and quantitative analysis of proteins and nucleic acids in a variety of sample matrices. This system can perform up to 50 tests in a single well, greatly reducing sample input, reagents, and labor to increase productivity. The Magpix system features an innovative design based on CCD imaging technology that allows for a more compact, robust system. It’s also easy to operate and is supported by a broad menu of commercially available assay kits, as well as reagents to build your assays.
NanoDrop™ OneOneC Microvolume UV Vis Spectrophotometer
Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometers quantify and qualify DNA, RNA, and proteins with only 1-2 μL in seconds. NanoDrop One spectrophotometer is built with Thermo Scientific™ Acclaro™ Sample Intelligence technology that helps you understand the quality of your sample before using it in downstream applications. With contaminant analysis, sample information alerts, and on-demand technical support, the Acclaro technology brings a new level of confidence in results, making NanoDrop One the ideal UV-Vis spectrophotometer for life science researchers.
Nikon Confocal Microscope
The ECLIPSE Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput. The Ti2’s exceptionally stable, drift-free platform is designed to meet the demands of super-resolution imaging while its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2’s unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility.
Motorized and intelligent model for advanced imaging applications. Compatible with PFS, auto-correction collar, and external phase contrast system. The base of choice for live-cell imaging, high-content applications, confocal, and super-resolution.
In combination with Nikon’s powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging.
Synergy H1 Biotek Microplate Reader
Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader.
The monochromator optics uses a third-generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance, and high-performance luminescence detection.
UVP ChemStudio Touch Series – Western Blot Imager
No matter what your preferred method of western blotting is, the Analytik Jena UVP ChemStudio Series features the highest sensitivity in gel analysis and blotting available. Fluorescent western blotting is the preferred method for quantitative comparisons between protein samples and for detection of multiple proteins. However, the ChemStudio and ChemStudio PLUS systems were built with flexibility in mind, providing RGB as standard and NIR modules with a laser-based source at two channels: 660 nm and 787 nm detection as optional.
Whatever your preferred research method, the UVP ChemStudio product family was built to streamline your protocol from detection to analysis, providing the most accurate quantitation of data for an unlimited range of applications.
The UVP ChemStudio gel and blot imagers feature the newest technology, a brand-new software interface, and top-of-the-line camera options that guarantee a wider dynamic range of imaging than ever before. With a ChemStudio in your lab, your research is unlimited.
Varioskan™ LUX multimode microplate reader
Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers’ best interest.
The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.
Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.
Zeiss Axiovert 200M Fluorescence Microscope
The Zeiss Axiovert 200M Fluorescence Microscope is a motorized inverted microscope with an AxioCam MRc5 color camera and a Hamamatsu ORCA-ER monochrome camera controlled by AxioVision 4.8.2 software. This microscope is well-equipped for transmitted light microscopy and fluorescence microscopy.
Luminex 200
The department houses a LX200. For instrument training and reservations, please contact Shannon Wallet, Ph.D., Associate Professor in Oral Biology, For technical information about the LX200, please visit the following links: