Ana Duran-Pinedo, Ph.D.
Research Assistant Professor
Department of Oral Biology
1395 Center Drive, DG-39
Gainesville, FL, 32610-0424
• Ph. D. Microbiology, University of Barcelona. Spain, 2001
• B.Sc. Bacteriology, University Javeriana. Colombia, 1994
My research main interest is to understand the role of Tannerella forsythia on the development of periodontal disease with particular focus on the study of new putative virulence factors. T. forsythia is a major periodontal pathogen strongly associated with periodontitis and a member of the Red complex (Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola). It was first isolated at Forsyth Institute by Anne Tanner and Sig Socransky, and named in recognition to its discoverers and the Institute. As standpoint, I have studied the gene expression profiles of oral communities on progressing and stablished periodontitis using Metatranscriptomic analysis. I have focused my research on understanding what is happening to T.forsythia, in the real context of the complex oral microbial communities and the host. We have found that during disease progression T.Forsythia is expressing at high level a protein causing collagen destruction in the tooth supporting structures. Collagen is the main component on the periodontium and its degradation lead to the loss of tooth, decreasing the quality of life of the patient. For the last 14 years I have been working on the biology of the anaerobes. As a postdoctoral fellow at University of Illinois at Champaign-Urbana, I carried out research on in vivo recombination of Bacteroides conjugative transposon CtnDOT, a genetic element involved in the spread of erythromycin resistance. Later on, I moved to The Forsyth Institute, where I started my research on the study of the genes controlled by Two Component Systems most in P.gingivalis.
As mentioned above, currently, the main focus of my research is understanding the contribution of Tannerella forsythia to the progression and development of periodontitis
NIH (NIDCR, R03 DE023394) “Highly up regulated proteins in Tannerella forsythia during periodontal disease”
Yost S, Duran-Pinedo AE, Krishnan K, Frias-Lopez J. 2017. Potassium is a key signal in host-microbiome dysbiosis in periodontitis. PloS Pathog. 13(6): e1006457. PMID: 28632755
Yost S and Duran-Pinedo AE. 2017.Contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen. Infect and Immun. Under review.
Duran-Pinedo AE, Frias-Lopez J. 2015. Beyond microbial community composition: functional activities of the oral microbiome in health and disease. Microbes Infect. 17(7):505-16. PMID: 25862077. PMCID: PMC4495649
Duran-Pinedo AE, Yost S, Frias-Lopez J. 2015. Small RNA Transcriptome of the Oral Microbiome during Periodontitis Progression. Appl Environ Microbiol. 81(19):6688-99. PMID: 26187962: PMCID: PMC4561685.
Yost S, Duran-Pinedo AE, Teles R, Krishnan K, Frias-Lopez J. 2015. Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. Genome Med. 7(1):27. PMID: 25918553; PMCID: PMC4410737
Duran-Pinedo AE, Chen T, Teles R, Starr JR, Wang X, Krishnan K, Frias-Lopez J. 2014. Community-wide transcriptome of the oral microbiome in subjects with and without periodontitis. ISME J. 8(8):1659-72. PMID:24599074; NIHMSID: NIHMS558378.
Duran-Pinedo AE, Baker VD, Frias-Lopez J. 2014. The periodontal pathogen Porphyromonas gingivalis induces expression of transposases and cell death of Streptococcus mitis in a biofilm model. Infect Immun. 82(8):3374-82. PMID:24866802; PMCID: PMC4136200
Scott JC, Klein BA, Duran-Pinedo AE, Hu L, Duncan MJ. 2013. A two-component system regulates hemin acquisition in Porphyromonas gingivalis. PLoS One. Sep 5;8(9). PMID: 24039921.
Frias-Lopez J, Duran-Pinedo A. 2012. Effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model. J Bacteriol. 194(8):2082-95. PMID: 22328675
Duran-Pinedo AE, Paster B, Teles R, Frias-Lopez J. 2011.Correlation network analysis applied to complex biofilm communities.PLoS One. 2011;6(12):e28438. doi: 10.1371/journal.pone.0028438. PMID:22163302.
Duran-Pinedo AE, Nishikawa K, Duncan MJ. 2007.The RprY response regulator of Porphyromonas gingivalis. Mol Microbiol. May;64(4):1061-74. PMID: 17501928
Lucena F, Ribas F, Duran AE, Skraber S, Gantzer C, Campos C, Morón A, Calderón E, Jofre J.2006. Ocurrence of bacterial indicators and bacteriophages infecting enteric bacteria in groundwater in different geographical areas. J Appl Microbiol. 2006 Jul;101(1):96-102.
Lucena F, Duran AE, Morón A, Calderón E, Campos C, Gantzer C, Skraber S, Jofre J. 2004. Reduction of bacterial indicators and bacteriophages infecting faecal bacteria in primary and secondary wastewater treatments. J Appl Microbiol 97(5):1069-76. PMID: 15479424
Durán AE, Muniesa M, Mocé-Llivina L, Campos C, Jofre J, Lucena F. 2003. Usefulness of different groups of bacteriophages as model micro-organisms for evaluating chlorination. J Appl Microbiol.;95(1):29-37. PMID: 12807451
Durán AE, Muniesa M, Méndez X, Valero F, Lucena F, Jofre J. Removal and inactivation of
indicator Bacteriophages in fresh waters. 2002. J Appl Microbiol.;92(2):338-47. PMID:1184936
Schaper M, Durán AE, Jofre J. Comparative resistance of phages isolates of four genotypes of f-specific RNA bacteriophages to various inactivation processes.2002. Appl Environ Microbiol. Aug;68(8):3702-7. PMID: 12147462