Jacqueline Abranches, Ph.D.

Abranches_Jacqueline_webAssistant Professor

Department of Oral Biology
1395 Center Drive, D5-33B
Box 100424
Gainesville, FL 32610-0424
Phone: 352-273-6672
E-Mail: jabranches@dental.ufl.edu

  • D., Federal University of Rio de Janeiro, Brazil. Microbiology and Immunology. 2000
  • Sc., Federal University of Rio de Janeiro, Brazil. Microbiology and Immunology. 1995.
  • S. Federal University of Rio de Janeiro, Brazil. Biology – Ecology. 1992.

 

Research Interests:

  • Bacteria-Host Interactions
  • Systemic diseases
  • Oral Infections
  • Intracellular Bacteria
  • Biofilms
  • Bacterial stress tolerance

 

Main interests:

My main research is on microbe-host interactions with a particular focus on the role of bacterial adhesins in colonization of and persistence within host tissues, systemic infections and disease. I use physiology and molecular biology in combination with in vitro, ex vivo and in vivo approaches to better characterize the contribution of these adhesins to bacterial virulence and disease. Using Streptococcus mutans, as a model organism, my laboratory has characterized a protein involved in colonization and persistence outside of the oral cavity. In addition, we are also interested in understanding  how  enterococci,  streptococci and lactobacilli regulate their physiology in order to thrive in adverse conditions commonly encountered in their habitats such as acidic pH, low oxygen tension and low nutrient availability.

Publications:

  • The CtsR regulator controls the expression of clpC, clpE and clpP and is required for the virulence of Enterococcus faecalis in an invertebrate model. Cassenego AP, de Oliveira NE, Laport MS, Abranches J, Lemos JA, Giambiagi-deMarval M. Antonie Van Leeuwenhoek. 2016 Sep;109(9):1253-9. doi: 10.1007/s10482-016-0727-0. PMID: 27388279
  • Heterologous expression of Streptococcus mutans Cnm in Lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence. Freires IA, Avilés-Reyes A, Kitten T, Simpson-Haidaris PJ, Swartz M, Knight PA, Rosalen PL, Lemos JA, Abranches J. Virulence. 2016 Jun 3:1-12. [Epub ahead of print] PMID: 27260618
  • Inactivation of the spxA1 or spxA2 gene of Streptococcus mutans decreases virulence in the rat caries model. Galvão LC, Rosalen PL, Rivera-Ramos I, Franco GC, Kajfasz JK, Abranches J, Bueno-Silva B, Koo H, Lemos JA. Mol Oral Microbiol. 2016 Apr 1. doi: 10.1111/omi.12160. [Epub ahead of print] PMID: 27037617
  • The Collagen Binding Proteins of Streptococcus mutans and Related Streptococci. Avilés-Reyes A, Miller JH, Lemos JA, Abranches J. Mol Oral Microbiol. 2016 Mar 15. doi: 10.1111/omi.12158. [Epub ahead of print] Review. PMID: 26991416
  • Transcriptional profile of glucose-shocked and acid-adapted strains of Streptococcus mutans. Baker JL, Abranches J, Faustoferri RC, Hubbard CJ, Lemos JA, Courtney MA, Quivey R Jr. Mol Oral Microbiol. 2015 Dec;30(6):496-517. doi: 10.1111/omi.12110. PMID: 26042838
  • Transcriptional and Phenotypic Characterization of Novel Spx-Regulated Genes in Streptococcus mutans. Galvão LC, Miller JH, Kajfasz JK, Scott-Anne K, Freires IA, Franco GC, Abranches J, Rosalen PL, Lemos JA. PLoS One. 2015 Apr 23;10(4):e0124969. doi: 10.1371/journal.pone.0124969. PMID: 25905865
  • Kajfasz JK, Rivera-Ramos I, Scott-Anne K, Gregoire S, Abranches J, et al.Transcription of Oxidative Stress Genes Is Directly Activated by SpxA1 and, to a Lesser Extent, by SpxA2 in Streptococcus mutans. Journal of bacteriology. 2015; 197(13):2160-70.
  • Miller JH, Avilés-Reyes A, Scott-Anne K, Gregoire S, Watson GE, et al. The collagen binding protein Cnm contributes to oral colonization and cariogenicity of Streptococcus mutans OMZ175. Infection and immunity. 2015; 83(5):2001-10.
  • Galvão LC, Miller JH, Kajfasz JK, Scott-Anne K, Freires IA, et al.Transcriptional and Phenotypic Characterization of Novel Spx-Regulated Genes in Streptococcus mutans. PloS one. 2015; 10(4):e0124969.
  • Avilés-Reyes A, Miller JH, Simpson-Haidaris PJ, Hagen FK, Abranches J, et al.Modification of Streptococcus mutans Cnm by PgfS contributes to adhesion, endothelial cell invasion, and virulence. Journal of bacteriology. 2014; 196(15):2789-97.
  • Avilés-Reyes A, Miller JH, Simpson-Haidaris PJ, Lemos JA, Abranches J. Cnm is a major virulence factor of invasive Streptococcus mutans and part of a conserved three-gene locus. Molecular oral microbiology. 2014; 29(1):11-23.
  • Abranches J, Tijerina P, Avilés-Reyes A, Gaca AO, Kajfasz JK, et al. The cell wall-targeting antibiotic stimulon of Enterococcus faecalis. PloS one. 2014; 8(6):e64875.
  • Gaca AO, Kajfasz JK, Miller JH, Liu K, Wang JD, et al. Basal levels of (p)ppGpp in Enterococcus faecalis: the magic beyond the stringent response. mBio. 2013; 4(5):e00646-13.
  • Lemos JA, Quivey RG Jr, Koo H, Abranches J. Streptococcus mutans: a new Gram-positive paradigm? Microbiology (Reading, England). 2013; 159(Pt 3):436-45.
  • Bitoun JP, Liao S, McKey BA, Yao X, Fan Y, et al. Psr is involved in regulation of glucan production, and double deficiency of BrpA and Psr is lethal in Streptococcus mutans. Microbiology (Reading, England). 2013; 159(Pt 3):493-506.
  • Palmer SR, Miller JH, Abranches J, Zeng L, Lefebure T, et al. Phenotypic heterogeneity of genomically-diverse isolates of Streptococcus mutans. PloS one.2013; 8(4):e61358.
  • Gaca AO, Abranches J, Kajfasz JK, Lemos JA. Global transcriptional analysis of the stringent response in Enterococcus faecalis. Microbiology (Reading, England).2012; 158(Pt 8):1994-2004.
  • Kajfasz JK, Mendoza JE, Gaca AO, Miller JH, Koselny KA, et al. The Spx regulator modulates stress responses and virulence in Enterococcus faecalis. Infection and immunity. 2012; 80(7):2265-75.
  • Bitoun JP, Liao S, Yao X, Ahn SJ, Isoda R, et al. BrpA is involved in regulation of cell envelope stress responses in Streptococcus mutans. Applied and environmental microbiology. 2012; 78(8):2914-22.
  • Kajfasz JK, Abranches J, Lemos JA. Transcriptome analysis reveals that ClpXP proteolysis controls key virulence properties of Streptococcus mutans.Microbiology (Reading, England). 2011; 157(Pt 10):2880-90.
  • Abranches J, Miller JH, Martinez AR, Simpson-Haidaris PJ, Burne RA, et al. The collagen-binding protein Cnm is required for Streptococcus mutans adherence to and intracellular invasion of human coronary artery endothelial cells. Infection and immunity. 2011; 79(6):2277-84.
  • de Oliveira NE, Abranches J, Gaca AO, Laport MS, Damaso CR, et al. clpB, a class III heat-shock gene regulated by CtsR, is involved in thermotolerance and virulence of Enterococcus faecalis. Microbiology (Reading, England). 2011; 157(Pt 3):656-65.
  • Wen ZT, Nguyen AH, Bitoun JP, Abranches J, Baker HV, et al. Transcriptome analysis of LuxS-deficient Streptococcus mutans grown in biofilms. Molecular oral microbiology. 2011; 26(1):2-18.
  • Martinez AR, Abranches J, Kajfasz JK, Lemos JA. Characterization of the Streptococcus sobrinus acid-stress response by interspecies microarrays and proteomics. Molecular oral microbiology. 2010; 25(5):331-42.
  • Kajfasz JK, Rivera-Ramos I, Abranches J, Martinez AR, Rosalen PL, et al. Two Spx proteins modulate stress tolerance, survival, and virulence in Streptococcus mutans. Journal of bacteriology. 2010; 192(10):2546-56.
  • Lemos JA, Abranches J, Koo H, Marquis RE, Burne RA. Protocols to study the physiology of oral biofilms. Methods in molecular biology (Clifton, N.J.). 2010; 666:87-102.
  • Simpson-Haidaris PJ, Pollock SJ, Ramon S, Guo N, Woeller CF, et al. Anticancer Role of PPARgamma Agonists in Hematological Malignancies Found in the Vasculature, Marrow, and Eyes. PPAR research. 2010; 2010:814609.
  • Senadheera D, Krastel K, Mair R, Persadmehr A, Abranches J, et al. Inactivation of VicK affects acid production and acid survival of Streptococcus mutans.Journal of bacteriology. 2009; 191(20):6415-24.
  • Abranches J, Zeng L, Bélanger M, Rodrigues PH, Simpson-Haidaris PJ, et al.Invasion of human coronary artery endothelial cells by Streptococcus mutans OMZ175. Oral microbiology and immunology. 2009; 24(2):141-5.
  • Kajfasz JK, Martinez AR, Rivera-Ramos I, Abranches J, Koo H, et al. Role of Clp proteins in expression of virulence properties of Streptococcus mutans. Journal of bacteriology. 2009; 191(7):2060-8.
  • Abranches J, Martinez AR, Kajfasz JK, Chávez V, Garsin DA, et al. The molecular alarmone (p)ppGpp mediates stress responses, vancomycin tolerance, and virulence in Enterococcus faecalis. Journal of bacteriology. 2009; 191(7):2248-56.
  • Burne RA, Ahn SJ, Wen ZT, Zeng L, Lemos JA, et al. Opportunities for disrupting cariogenic biofilms. Advances in dental research. 2009; 21(1):17-20.
  • Lemos JA, Nascimento MM, Lin VK, Abranches J, Burne RA. Global regulation by (p)ppGpp and CodY in Streptococcus mutans. Journal of bacteriology. 2008; 190(15):5291-9.
  • Abranches J, Nascimento MM, Zeng L, Browngardt CM, Wen ZT, et al. CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. Journal of bacteriology. 2008; 190(7):2340-9.
  • Nascimento MM, Lemos JA, Abranches J, Lin VK, Burne RA. Role of RelA of Streptococcus mutans in global control of gene expression. Journal of bacteriology. 2008; 190(1):28-36.
  • Lemos JA, Lin VK, Nascimento MM, Abranches J, Burne RA. Three gene products govern (p)ppGpp production by Streptococcus mutans. Molecular microbiology. 2007; 65(6):1568-81.
  • Hasona A, Zuobi-Hasona K, Crowley PJ, Abranches J, Ruelf MA, et al. Membrane composition changes and physiological adaptation by Streptococcus mutans signal recognition particle pathway mutants. Journal of bacteriology. 2007; 189(4):1219-30.
  • Abranches J, Candella MM, Wen ZT, Baker HV, Burne RA. Different roles of EIIABMan and EIIGlc in regulation of energy metabolism, biofilm development, and competence in Streptococcus mutans. Journal of bacteriology. 2006; 188(11):3748-56.
  • Abranches J, Lemos JA, Burne RA. Osmotic stress responses of Streptococcus mutans UA159. FEMS microbiology letters. 2006; 255(2):240-6.
  • Koo H, Seils J, Abranches J, Burne RA, Bowen WH, et al. Influence of apigenin on gtf gene expression in Streptococcus mutans UA159. Antimicrobial agents and chemotherapy. 2006; 50(2):542-6.
  • Lemos JA, Brown TA Jr, Abranches J, Burne RA. Characteristics of Streptococcus mutans strains lacking the MazEF and RelBE toxin-antitoxin modules. FEMS microbiology letters. 2005; 253(2):251-7.
  • Lemos JA, Abranches J, Burne RA. Responses of cariogenic streptococci to environmental stresses. Current issues in molecular biology. 2005; 7(1):95-107.
  • Abranches J, Chen YY, Burne RA. Galactose metabolism by Streptococcus mutans.Applied and environmental microbiology. 2004; 70(10):6047-52.
  • Nascimento MM, Lemos JA, Abranches J, Gonçalves RB, Burne RA. Adaptive acid tolerance response of Streptococcus sobrinus. Journal of bacteriology. 2004; 186(19):6383-90.
  • Abranches J, Chen YY, Burne RA. Characterization of Streptococcus mutans strains deficient in EIIAB Man of the sugar phosphotransferase system. Applied and environmental microbiology. 2003; 69(8):4760-9.
  • Koo H, Pearson SK, Scott-Anne K, Abranches J, Cury JA, et al. Effects of apigenin and tt-farnesol on glucosyltransferase activity, biofilm viability and caries development in rats. Oral microbiology and immunology. 2002; 17(6):337-43.
  • Phan TN, Nguyen PT, Abranches J, Marquis RE. Fluoride and organic weak acids as respiration inhibitors for oral streptococci in acidified environments. Oral microbiology and immunology. 2002; 17(2):119-24.
  • Nguyen PT, Abranches J, Phan TN, Marquis RE. Repressed respiration of oral streptococci grown in biofilms. Current microbiology. 2002; 44(4):262-6.
  • Abranches J, Starmer WT, Hagler AN. Yeast-Yeast Interactions in Guava and Tomato Fruits. Microbial ecology. 2001; 42(2):186-192.
  • Yeast diversity and killer activity dispersed in fecal pellets from marsupials and rodents in a Brazilian tropical habitat mosaic Abranches,Jacqueline,, Valente,Patricia,, Nobrega,Hilda,N, Fernandez,Fernando,AS, Mendonca-Hagler,Leda,C, et al.FEMS microbiology ecology. 1998 May; 26(1):27-33.
  • Abranches J, Morais PB, Rosa CA, Mendonça-Hagler LC, Hagler AN. The incidence of killer activity and extracellular proteases in tropical yeast communities. Canadian journal of microbiology. 1997; 43(4):328-36.